Proteins secreted by vascular smooth muscle cells as substrates of lysyl oxidase.
Connect Tissue Res
; 40(2): 123-9, 1999.
Article
em En
| MEDLINE
| ID: mdl-10761637
ABSTRACT
The mixture of proteins secreted by neonatal rat aorta smooth muscle cells cultured in the presence of beta-aminopropionitrile was readily oxidized and polymerized upon incubation with purified or crude preparations of lysyl oxidase. Western blot analysis indicated that these substrates included 30-60kDa protein bands reactive with anti-elastin, presumed to be fragments derived from tropoelastin. Thus, truncated, elastin-like as well as other proteins accumulate in the media of these cultures which, in toto, can serve as a conveniently prepared, highly efficient substrate for the routine assay of lysyl oxidase activity.
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Base de dados:
MEDLINE
Assunto principal:
Elastina
/
Proteína-Lisina 6-Oxidase
/
Proteínas Musculares
/
Músculo Liso Vascular
Idioma:
En
Ano de publicação:
1999
Tipo de documento:
Article