Dynamic chromatin remodeling in the vicinity of J chain gene for the regulation of two stage-specific genes during B cell differentiation.
Mol Cells
; 10(1): 32-7, 2000 Feb 29.
Article
em En
| MEDLINE
| ID: mdl-10774744
ABSTRACT
Dynamic chromatin remodeling during B cell differentiation was identified in the vicinity of J chain gene. In pre-B cells, the enhancer-containing DNase I hypersensitive sites (HSSs) 3-4 were open. However, these HSSs 3-4 turned out to be unassociated with J chain gene expression, as the J chain promoter-containing HSS1 remained in a closed state. The open enhancer HSSs 3-4 in the pre-B cells might be related to the expression of a pre-B cell-specific gene upstream of the HSSs 3-4, which was identified in our Northern blot analyses. The HSSs 3-4 are then closed in the next immature and mature B cell stages until the IL-2 opens the HSSs 3-4 again as well as HSS1 to express J chain gene in the primary immune responses. The dynamic regulation of chromatin structure during B cell differentiation for the expression of two stage-specific genes will provide a good model system for the study of B cell differentiation and gene expression.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Cromatina
/
Cadeias J de Imunoglobulina
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article