Assessment of flhDC mRNA levels in Serratia liquefaciens swarm cells.
J Bacteriol
; 182(10): 2680-6, 2000 May.
Article
em En
| MEDLINE
| ID: mdl-10781533
ABSTRACT
We reported previously that artificial overexpression of the flhDC operon in liquid-grown Serratia liquefaciens resulted in the formation of filamentous, multinucleated, and hyperflagellated cells that were indistinguishable from surface-induced swarm cells (L. Eberl, G. Christiansen, S. Molin, and M. Givskov, J. Bacteriol. 178554-559, 1996). In the present report we show by means of reporter gene measurements, Northern analysis, and in situ reverse transcription-PCR that the amount of flhDC mRNA in surface-grown swarm cells does not exceed the maximum level found in nondifferentiated, vegetative cells. This suggests that surface-induced S. liquefaciens swarm cell differentiation, although dependent on flhDC gene expression, does not occur through elevated flhDC mRNA levels.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Serratia
/
Transativadores
/
Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article