The Drosophila SHC adaptor protein is required for signaling by a subset of receptor tyrosine kinases.
Mol Cell
; 5(2): 231-41, 2000 Feb.
Article
em En
| MEDLINE
| ID: mdl-10882065
ABSTRACT
Receptor tyrosine kinases (RTKs) transduce signals via cytoplasmic adaptor proteins to downstream signaling components. We have identified loss-of-function mutations in dshc, the Drosophila homolog of the mammalian adaptor protein SHC. A point mutation in the phosphotyrosine binding (PTB) domain completely abolishes DSHC function and provides in vivo evidence for the function of PTB domains. Unlike other adaptor proteins, DSHC is involved in signaling by only a subset of RTKs dshc mutants show defects in Torso and DER but not Sevenless signaling, which is confirmed by epistasis experiments. We show by double-mutant analysis that the adaptors DOS, DRK, and DSHC act in parallel to transduce the Torso signal. Our results suggest that DSHC confers specificity to receptor signaling.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Quinases
/
Receptores Proteína Tirosina Quinases
/
Proteínas de Insetos
/
Proteínas de Drosophila
/
Proteínas Adaptadoras de Transporte Vesicular
/
Proteínas Adaptadoras de Transdução de Sinal
/
Drosophila
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article