Cloning and recombinant expression of the La RNA-binding protein from Trypanosoma brucei.
Biochim Biophys Acta
; 1492(2-3): 483-7, 2000 Jul 24.
Article
em En
| MEDLINE
| ID: mdl-10899586
ABSTRACT
We report the isolation, cloning and recombinant expression of a Trypanosoma brucei homolog of the La RNA-binding protein. Based on peptide sequence information we have isolated a cDNA clone which encodes a protein of 335 amino acids with a predicted molecular weight of 37.7 kDa. The amino acid sequence fits the domain structure of known La proteins and contains a putative ATP-binding site located in the COOH-terminal domain. The cDNA was expressed as a glutathione S-transferase fusion protein in Escherichia coli, and the recombinant protein displayed RNA-binding activity in an electrophoretic mobility shift assay.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Ribonucleoproteínas
/
Autoantígenos
/
Trypanosoma brucei brucei
/
Proteínas de Protozoários
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article