A chromatin insulator protects retrovirus vectors from chromosomal position effects.
Proc Natl Acad Sci U S A
; 97(16): 9150-5, 2000 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-10908661
ABSTRACT
Recombinant murine retroviruses are widely used as delivery vectors for gene therapy. However, once integrated into a chromosome, these vectors often suffer from profound position effects, with vector silencing observed in vitro and in vivo. To overcome this problem, we investigated whether the HS4 chromatin insulator from the chicken beta-globin locus control region could protect a retrovirus vector from position effects. When used to flank a reporter vector, this element significantly increased the fraction of transduced cells that expressed the provirus in cultures and in mice transplanted with transduced marrow. These results demonstrate that a chromatin insulator can improve the expression performance of a widely used class of gene therapy vectors by protecting these vectors from chromosomal position effects.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Retroviridae
/
Cromatina
/
Cromossomos
/
Vetores Genéticos
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article