Echinococcus granulosus: DNA extraction from germinal layers allows strain determination in fertile and nonfertile hydatid cysts.
Exp Parasitol
; 95(2): 122-7, 2000 Jun.
Article
em En
| MEDLINE
| ID: mdl-10910713
ABSTRACT
A method for the isolation of Echinococcus granulosus DNA from germinal layers of hydatid cysts is described. The method includes a hexadecyltrimethylammonium bromide/chloroform extraction and an adsorption to diatomaceous earth suspension. DNA suitable for polymerase chain reaction was obtained and used for parasite strain determination by mitochondrial cytochrome c oxidase I gene sequencing. Fertile and nonfertile cyst isolates from sheep, cattle, pigs, and humans were characterized. Hitherto, no direct parasite strain characterization has been made on nonfertile hydatid cysts, whereas here we report that nonfertile hydatid cysts were produced by sheep strain (G1 genotype) in sheep, cattle, and humans and by pig strain (G7 genotype) in pigs.
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Base de dados:
MEDLINE
Assunto principal:
DNA de Helmintos
/
Equinococose
/
Echinococcus
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article