Cholestan-3beta,5alpha,6beta-triol, but not 7-ketocholesterol, suppresses taurocholate-induced mucin secretion by cultured dog gallbladder epithelial cells.
FEBS Lett
; 478(1-2): 113-8, 2000 Jul 28.
Article
em En
| MEDLINE
| ID: mdl-10922480
ABSTRACT
In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3beta,5alpha,6beta-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells. We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects in cultured human pulmonary artery endothelial cells (HPAE). Oxysterols inhibited cell proliferation in a dose-dependent fashion. Oxysterols inhibited cell growth to 50% of control at a higher dose for DGBE cells than for HPAE cells. TriolC was more cytotoxic than 7KC. We also investigated the effect of oxysterols on bile salt-induced mucin secretion by DGBE cells. TriolC suppressed mucin secretion by DGBE cells, whereas 7KC did not. These findings support the hypothesis that biliary oxysterols affect gallbladder mucosal function.
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Base de dados:
MEDLINE
Assunto principal:
Ácido Taurocólico
/
Colestanóis
/
Células Epiteliais
/
Vesícula Biliar
/
Cetocolesteróis
/
Mucinas
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article