Inducible nitric oxide synthase transcription in human lung transplantation.
Transplantation
; 70(2): 384-5, 2000 Jul 27.
Article
em En
| MEDLINE
| ID: mdl-10933168
ABSTRACT
BACKGROUND:
Recent animal data suggest that inducible nitric oxide synthase (iNOS) mRNA expression in the bronchoalveolar lavage (BAL) may be useful for the diagnosis of lung rejection. The aim of this study was to evaluate iNOS mRNA transcription in the BAL fluid of human lung allografts.METHODS:
iNOS mRNA transcription was quantified by competitive reverse transcription-polymerase chain reaction in 51 BAL cell pellets of lung transplant patients. According to bacteriological and histological results, BAL samples were divided into three groups normal (n=21), acute rejection (AR, n=15), and infection (INF, n=15).RESULTS:
Compared with the control group, iNOS transcription increased significantly with INF (P=0.0005) but only slightly with AR (P>0.05). INF values were significantly higher than AR values (P=0.0029).CONCLUSION:
BAL iNOS mRNA transcript determination by competitive reverse transcription-polymerase chain reaction may be useful in differentiating infected from normal and/or acutely rejecting allografts.
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Base de dados:
MEDLINE
Assunto principal:
Transplante de Pulmão
/
Óxido Nítrico Sintase
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article