Imaging Tc-99m-labeled FGF-1 targeting in rats.
Nucl Med Biol
; 27(4): 407-14, 2000 May.
Article
em En
| MEDLINE
| ID: mdl-10938477
ABSTRACT
Recombinant human acidic fibroblast growth factor (FGF-1) was radiolabeled with (99m)Tc by the HYNIC method. The (99m)Tc-FGF-1 retained its representative molecular mass, heparin affinity, cellular binding to both low (Kd = 9.5 nM) and high (Kd = 125 pM) affinity sites, and mitogenic activity. Gamma camera imaging after intravenous dosing in rats confirmed high liver and kidney binding. Heparin significantly decreased (99m)Tc-FGF-1 liver uptake and increased urinary excretion. These studies illustrate a new method for imaging FGF-1 targeting under various conditions.
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Base de dados:
MEDLINE
Assunto principal:
Fator 2 de Crescimento de Fibroblastos
/
Tecnécio
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article