Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non-artificial conditions.
Yeast
; 16(12): 1107-10, 2000 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-10953082
ABSTRACT
Transformants of bakers' yeast (Saccharomyces cerevisiae) can be generated when non-growing cells metabolize sugars (without additional nutrients) in the presence of plasmid DNA. These results suggest that there is a mechanism by which DNA can naturally be taken up by the yeast cell. Natural transformation does not take place in common complete or minimal yeast culture media such as YPD and YNB. The starvation conditions used in our experiments thus seem to be an important prerequisite for such transformation events.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Sacarose
/
Transformação Genética
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article