Turnover of protein synthetic elongation and initiation factors in Escherichia coli.
J Biol Chem
; 250(12): 4714-7, 1975 Jun 25.
Article
em En
| MEDLINE
| ID: mdl-1095575
ABSTRACT
In an effort to understand the regulation of expression of the factors required for protein biosynthesis, we have measured the turnover of elongation factor G (EF-G) and initiation factor 2 (IF-2). Using a quantitative assay which involves immunoprecipitation and gel electrophoresis, it was found that both of these factors are very stable cellular proteins, but that their rates of accumulation are quite different, indicating a differential rate of synthesis. The effects of amino acid starvation and the relaxed response were also examined. The results showed that under these conditions EF-G and IF-2 are neither synthesized nor degraded.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Fatores de Alongamento de Peptídeos
/
Fatores de Iniciação de Peptídeos
/
Escherichia coli
Idioma:
En
Ano de publicação:
1975
Tipo de documento:
Article