A region of the myosin rod important for interaction with paramyosin in Caenorhabditis elegans striated muscle.
Genetics
; 156(2): 631-43, 2000 Oct.
Article
em En
| MEDLINE
| ID: mdl-11014812
ABSTRACT
The precise arrangement of molecules within the thick filament, as well as the mechanisms by which this arrangement is specified, remains unclear. In this article, we have exploited a unique genetic interaction between one isoform of myosin heavy chain (MHC) and paramyosin in Caenorhabditis elegans to probe the molecular interaction between MHC and paramyosin in vivo. Using chimeric myosin constructs, we have defined a 322-residue region of the MHC A rod critical for suppression of the structural and motility defects associated with the unc-15(e73) allele. Chimeric constructs lacking this region of MHC A either fail to suppress, or act as dominant enhancers of, the e73 phenotype. Although the 322-residue region is required for suppression activity, our data suggest that sequences along the length of the rod also play a role in the isoform-specific interaction between MHC A and paramyosin. Our genetic and cell biological analyses of construct behavior suggest that the 322-residue region of MHC A is important for thick filament stability. We present a model in which this region mediates an avid interaction between MHC A and paramyosin in parallel arrangement in formation of the filament arms.
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Base de dados:
MEDLINE
Assunto principal:
Tropomiosina
/
Miosinas
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Caenorhabditis elegans
/
Músculo Esquelético
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article