Human p32: a coactivator for Epstein-Barr virus nuclear antigen-1-mediated transcriptional activation and possible role in viral latent cycle DNA replication.
Virology
; 275(1): 145-57, 2000 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-11017796
ABSTRACT
The Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA-1) is required for the maintenance of the viral chromosome in latently infected, proliferating cells and plays a role in latent cycle DNA replication. EBNA-1 also functions as a positive and negative regulator of EBV gene expression. We have investigated the interaction of EBNA-1 with p32, a host mitochondrial protein that associates with EBNA-1 in EBV-positive Burkitt's lymphoma cells. Using a chromatin immunoprecipitation assay, we found that a fraction of p32 localizes to the viral latent cycle origin of DNA replication oriP in vivo. p32 binds EBNA-1 independently of other proteins or DNA. EBNA-1 variants lacking one of two p32 binding elements did not interact stably with p32 in cultured cells and were defective for both transcriptional activation of a reporter gene linked to oriP FR and replication and/or maintenance of a plasmid bearing oriP. These results support a role for p32 in transcriptional activation by EBNA-1 and suggest that p32 plays a role in EBV latent cycle DNA replication.
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Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
/
Proteínas
/
Ativação Transcricional
/
Latência Viral
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Receptores de Hialuronatos
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Antígenos Nucleares do Vírus Epstein-Barr
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Replicação do DNA
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article