Induction of vascular endothelial growth factor receptors and phosphatidylinositol 3'-kinase/Akt signaling by global cerebral ischemia in the rat.
Neuroscience
; 100(4): 713-7, 2000.
Article
em En
| MEDLINE
| ID: mdl-11036205
ABSTRACT
Vascular endothelial growth factor is an angiogenic peptide that binds to tyrosine kinase receptors on target cells to activate signal transduction pathways involving phosphatidylinositol 3'-kinase and the serine-threonine protein kinase, Akt. To determine whether this signaling pathway is activated in cerebral ischemia, we examined the expression of vascular endothelial growth factor receptors 1 and 2, and phosphatidylinositol 3'-kinase-activated phospho-Akt, in the cerebral cortex and hippocampus following transient global cerebral ischemia in the rat. Western blot analysis and immunocytochemistry demonstrated induction of vascular endothelial growth factor receptor 1 and 2 expression, and of anti-phosphatidylinositol 3'-kinase-immunoprecipitated phospho-Akt, in vulnerable regions of the cortex and hippocampus after 15 min of global ischemia and 4-72 h of reperfusion. These findings demonstrate that vascular endothelial growth factor receptors and receptor-coupled signal transduction pathways are induced in ischemic brain in vivo, and could therefore participate in endogenous neuroprotective responses to ischemia.
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Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
/
Ataque Isquêmico Transitório
/
Proteínas Proto-Oncogênicas
/
Proteínas Serina-Treonina Quinases
/
Receptores de Fatores de Crescimento
/
Receptores Proteína Tirosina Quinases
/
Fosfatidilinositol 3-Quinases
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article