12-O-tetradecanoyl-phorbol-13-acetate down-regulates the Huntingtin promoter at Sp1 sites.
Neuroreport
; 11(14): 3157-61, 2000 Sep 28.
Article
em En
| MEDLINE
| ID: mdl-11043541
ABSTRACT
We have studied the effects of the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on Huntington's disease (HD) gene transcription in neuronal and non-neuronal cell lines, to investigate pathways regulating HD gene expression. TPA reduced transcription from the HD gene promoter in SK-N-SH (neuroblastoma) and HeLa cells but not in JEG3 (choriocarcinoma) cells. In SK-N-SH cells, the responsible cis-acting promoter sequences comprise the tandemly duplicated Sp1 sites in the region from -213 to -174, relative to the translation start site. The TPA-down-regulating region in HeLa cells was mapped to the sequence from -141 to -126. In conclusion, this demonstrates that HD gene transcription can be down-regulated in vitro in a cell-specific manner.
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Base de dados:
MEDLINE
Assunto principal:
Acetato de Tetradecanoilforbol
/
Proteínas Nucleares
/
Regulação para Baixo
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Regulação da Expressão Gênica
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Regiões Promotoras Genéticas
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Doença de Huntington
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Proteínas do Tecido Nervoso
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article