Unmodified calcium concentrations in tumour necrosis factor receptor subtype-mediated apoptotic cell death.
Mol Cell Biochem
; 211(1-2): 19-26, 2000 Aug.
Article
em En
| MEDLINE
| ID: mdl-11055543
ABSTRACT
Tumour necrosis factor-alpha (TNF) receptors mediate a variety of effects dependent on cell type. A role for Ca2+ in TNF-induced death remains uncertain. Here we investigated restricting intracellular/extracellular Ca2+ in HeLa epithelial carcinoma cells expressing low and high levels of p75TNFR receptor subtype and KYM-1 rhabdomyosarcoma cells, models of rapid TNF-induced apoptosis. Ca2+ -chelators EGTA and BAPTA-AM as well as microsomal Ca2+ -ATPase inhibitor thapsigargin, did not alter TNF-induced death. TNF was also unable to alter resting [Ca2+]i levels which remained < 200 nM even during times when these cells were undergoing apoptotic cell death. These findings indicate no role for modulated Ca2+ concentrations in TNF-induced apoptotic cell death.
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Base de dados:
MEDLINE
Assunto principal:
Antígenos CD
/
Sobrevivência Celular
/
Cálcio
/
Fura-2
/
Fator de Necrose Tumoral alfa
/
Apoptose
/
Receptores do Fator de Necrose Tumoral
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article