Episomal and stable expression of the luciferase reporter gene for quantifying Leishmania spp. infections in macrophages and in animal models.
Mol Biochem Parasitol
; 110(2): 195-206, 2000 Oct.
Article
em En
| MEDLINE
| ID: mdl-11071276
ABSTRACT
We have expressed the reporter firefly luciferase gene (LUC) in Leishmania donovani and Leishmania major either as part of episomal vectors or integrated into the parasite genome under the control of their respective ribosomal promoter regions. An excellent linear correlation between parasite number and luciferase activity was observed with all the transfectants. LUC-expressing recombinant parasites were useful to monitor Leishmania spp. infections in macrophages or in animal models. For prolonged growth in absence of drug selection, such as within animal models, quantitation of parasites is more reliable when the reporter gene LUC is stably integrated in the parasite genome. These recombinant strains should be useful tools to monitor Leishmania growth under a number of conditions.
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Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Leishmaniose
/
Leishmania major
/
Leishmania infantum
/
Genes Reporter
/
Luciferases
/
Macrófagos
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article