Regional differences in signalling transduction pathways among smooth muscle cells from rabbit colon.
Cell Signal
; 12(9-10): 683-9, 2000 Oct.
Article
em En
| MEDLINE
| ID: mdl-11080621
ABSTRACT
Smooth muscle cells (SMC) from the circular muscle layer of rabbit colon, taken from the proximal and distal regions that are known to have different physiological and motor activities, were used to highlight distinct regional intrinsic myogenic properties and to investigate the correlations between receptor and signalling transduction pathways. Contractile agonists were shown to be more potent on proximal than on distal SMC in inducing contraction and intracellular Ca(2+) increase. Concentration-response curves of agonists-induced Ca(2+) increase were constantly shifted to the right, though remaining parallel, with respect to contraction curves, independently of the region analysed. Using agents activating different steps of cAMP-or cGMP-mediated intracellular cascades, main regional differences were revealed as far as relaxation was concerned. Relaxation of proximal SMC was found to be essentially cGMP mediated, while that of distal SMC was cAMP mediated. In conclusion, the motor patterns of the two regions appear to be influenced by distinct regional biochemical characteristics that are intrinsic to colonic SMC.
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Base de dados:
MEDLINE
Assunto principal:
Colo
/
Sinalização do Cálcio
/
Músculo Liso
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article