Multicolor fluorescence in situ hybridization with peptide nucleic acid probes for enumeration of specific chromosomes in human cells.
Genes Chromosomes Cancer
; 30(1): 57-63, 2001 Jan.
Article
em En
| MEDLINE
| ID: mdl-11107176
ABSTRACT
In previous studies, we showed that peptide nucleic acid (PNA) probes have significant advantages over conventional synthetic RNA or DNA probes in FISH procedures for detecting telomeric and trinucleotide repeat sequences. Here, we report that directly labeled PNA probes recognizing chromosome-specific repeat sequences are also powerful tools for detecting and enumerating specific chromosomes in interphase and metaphase cells. This is illustrated by multicolor FISH experiments with cells from normal individuals and patients with numerical sex chromosome aberrations.
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Base de dados:
MEDLINE
Assunto principal:
Sondas de Ácido Nucleico
/
Cromossomos Humanos
/
Hibridização in Situ Fluorescente
/
Ácidos Nucleicos Peptídicos
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article