Kidney produces a novel acylated peptide, ghrelin.
FEBS Lett
; 486(3): 213-6, 2000 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-11119706
ABSTRACT
Ghrelin is a novel growth hormone-releasing peptide with a unique acylated structure. Here we reveal that prepro-ghrelin gene is expressed in the mouse kidney and glomerulus. We also show by reverse-phase high performance liquid chromatography coupled with radioimmunoassay that the mouse kidney does produce ghrelin. The ghrelin immunoreactivity in the mouse kidney is 6.79+/-0.48 fmol/mg (n=5), which is much more abundant than that in the mouse plasma of 0.339+/-0.029 fmol/microl (n=6). Furthermore, prepro-ghrelin gene is expressed in cultured rat mesangial cells, fibroblast-like NRK-49F cells and mouse podocytes, but not in rat epithelial cell-like NRK-52E cells. Ghrelin receptor gene is also expressed in the rat kidney. These findings demonstrate that the kidney, glomerulus and renal cells express prepro-ghrelin gene and ghrelin is produced locally in the kidney, and suggest the endocrine and/or paracrine roles of ghrelin in the kidney.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Hormônios Peptídicos
/
Receptores Acoplados a Proteínas G
/
Rim
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article