Inhibition of astrocyte TNFalpha expression by extracellular potassium.
Brain Res
; 888(1): 176-179, 2001 Jan 05.
Article
em En
| MEDLINE
| ID: mdl-11146066
ABSTRACT
TNFalpha and IL-6 are cytokines of great interest, given the numerous biological activities and the documented expression in several central nervous system (CNS) pathologies. In this report, we have examined cultures of IL-1- or IL-1/IFNgamma-activated human fetal astrocytes as a model to study mechanisms of cytokine regulation in the inflamed CNS. Since one of the major functions of astrocytes is spatial buffering of K(+) ions, we examined the effect of high extracellular KCl on astrocyte cytokine expression by ribonuclease protection assay and ELISA. Results demonstrate that astrocyte TNFalpha production was potently inhibited by K(+) with 44 and 89% inhibition at 25 and 55 mM K+, respectively. In contrast, astrocyte IL-6 inhibition required higher concentrations of K+ (>/=75 mM). These results demonstrate a novel role for astrocyte potassium channel activity in modulation of glial cytokine production.
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Base de dados:
MEDLINE
Assunto principal:
Cloreto de Potássio
/
Astrócitos
/
Fator de Necrose Tumoral alfa
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article