Pheochromocytoma cell lines from heterozygous neurofibromatosis knockout mice.
Cell Tissue Res
; 302(3): 309-20, 2000 Dec.
Article
em En
| MEDLINE
| ID: mdl-11151443
ABSTRACT
Transplantable tumors and cell lines have been developed from pheochromocytomas arising in mice with a heterozygous knockout mutation of the neurofibromatosis gene, Nf1. Nf1 encodes a ras-GTPase-activating protein, neurofibromin, and mouse pheochromocytoma (MPC) cells in primary cultures typically show extensive spontaneous neuronal differentiation that may result from the loss of the remaining wild-type allele and defective regulation of ras signaling. However, all MPC cell lines express neurofibromin, suggesting that preservation of the wild-type allele may be required to permit the propagation of MPC cells in vitro. MPC lines differ from PC12 cells in that they express both endogenous phenylethanolamine N-methyltransferase (PNMT) and full-length PNMT reporter constructs. PNMT expression is increased by dexamethasone and by cell-cell contact in suspension cultures. Mouse pheochromocytomas are a new tool for studying genes and signaling pathways that regulate cell growth and differentiation in adrenal medullary neoplasms and are a unique model for studying the regulation of PNMT expression.
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Base de dados:
MEDLINE
Assunto principal:
Feocromocitoma
/
Fatores de Transcrição
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Células Tumorais Cultivadas
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Genes da Neurofibromatose 1
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Neoplasias das Glândulas Suprarrenais
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article