Immune responses following simian/human immunodeficiency virus (SHIV) challenge of rhesus macaques after human immunodeficiency virus (HIV)-1 third variable domain (V3) loop-based genetic immunization.
J Med Primatol
; 29(6): 371-86, 2000 Dec.
Article
em En
| MEDLINE
| ID: mdl-11168828
ABSTRACT
Following DNA immunization of rhesus macaques with a plasmid encoding the human immunodeficiency virus (HIV)-1 third variable domain (V3) loop, presented by pseudo-viral envelope particles of hepatitis B virus, specific immune responses were induced. The primates were then inoculated with a chimeric simian/human immunodeficiency virus (SHIV). All the animals were infected, but the V3-specific immunization provided a relative attenuation of the acute phase of infection in the absence of neutralizing antibody. In all animals, SHIV-specific cytotoxic T-lymphocyte precursors (CTLp) were detected early in peripheral blood and lymph nodes. The viremia peak correlated significantly with the decrease in CD4+ T cells and with a transient increase in the percentage of natural killer cells. The infection induced an oligoclonalization of the CD8+ T-cell variable beta chain repertoire in the blood. Surprisingly, HIV envelope-specific CTLp generated by genetic immunization may be governed by distinct circulation rules compared to SHIV-specific CTLp induced by infection.
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Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Proteína gp120 do Envelope de HIV
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Síndrome de Imunodeficiência Adquirida dos Símios
/
HIV-1
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Vacinas contra a AIDS
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Macaca mulatta
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Anticorpos Antivirais
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article