Two cases of severe bronchopneumonia due to influenza A (H3N2) virus: detection of influenza virus gene using reverse transcription polymerase chain reaction.
Intern Med
; 40(1): 61-7, 2001 Jan.
Article
em En
| MEDLINE
| ID: mdl-11201374
ABSTRACT
We report two cases of severe bronchopneumonia due to influenza A (H3N2) virus. The severity of the disease necessitated initiation of empiric therapy based on the present illness and clinical data on admission. Both patients were improved by artificial ventilation with positive end-expiratory pressures and administration of broad spectrum antibiotics and corticosteroids before confirming the diagnosis of viral bronchopneumonia using viral culture and serological tests. Within 24 hours, influenza A (H3N2) virus was identified by amplification of the pathogen genes by reverse transcription polymerase chain reaction (RT-PCR) using the stored bronchoalveolar lavage (BAL) fluids of both cases. This suggests that a combination of detection methods of pathogens using RT-PCR and BAL fluid will facilitate determination of rational treatment aimed at influenza A virus.
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Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
/
Pneumonia Viral
/
Broncopneumonia
/
RNA Viral
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Vírus da Influenza A Subtipo H3N2
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article