Tissues and cells involved in the invasion of the rabbit intestinal tract by sporozoites of Eimeria coecicola.
Parasitol Res
; 87(2): 98-106, 2001 Feb.
Article
em En
| MEDLINE
| ID: mdl-11206118
ABSTRACT
This study was designed to identify an extra-intestinal route of migration of Eimeria coecicola sporozoites and the types of cell harbouring the parasite during the invasion of the intestine. The presence of E. coecicola in blood, spleen and mesenteric lymph nodes of infected donor rabbits was demonstrated by immunohistology on donor organs and measurement of oocyst excretion by coccidia-free recipient rabbits injected with whole-cell suspensions prepared from donor tissues. Two types of donor lymphocyte, B (IgM+) and T (CD5+), were labelled using a two-colour immunofluorescence-labelling technique and separated with a cell-sorter (FACStar(Plus)). The presence of parasites in the sorted cells was assessed by direct examination and by using the same in vivo test after intravenous injection of IgM+ B or CD5+ T lymphocytes collected from donors at different times after inoculation. This test provided evidence that the parasites were alive and still infectious within the sorted lymphocytes. It was demonstrated that both B and T lymphocytes were infected.
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Base de dados:
MEDLINE
Assunto principal:
Coccidiose
/
Eimeria
/
Intestinos
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article