EGF regulates a complex pattern of gene expression and represses smooth muscle differentiation during the neurotypic conversion of the neural-crest-derived TC-1S cell line.
Exp Cell Res
; 264(2): 353-62, 2001 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-11262192
ABSTRACT
EGF, known to sustain CNS neuronal progenitors, also promotes a neurotypic response in the thymic neural-crest-derived TC-1S cell line. We report here the use of TC-1S cells as a model to identify the genetic programs regulated during the neurotypic response induced by EGF and to isolate 23 EGF-responsive genes. Among them 5 represent novel cDNAs, while 18 are known genes, whose regulation by EGF is associated with the mitogenic or differentiating effects of the growth factor. The repression of smooth muscle alpha-actin and SM22alpha genes by EGF and their increase by TGFbeta suggest that the TC-1S line includes neural crest multipotent cells whose smooth muscle differentiation is repressed upon EGF treatment and stimulated by TGFbeta. Therefore, we identified a complex pattern of EGF-target genes and propose EGF as a novel signal able to recruit postmigratory neural-crest-derived cells along proliferation and cell lineage choice pathways.
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Base de dados:
MEDLINE
Assunto principal:
Perfilação da Expressão Gênica
/
Fator de Crescimento Epidérmico
/
Músculo Liso
/
Crista Neural
/
Neurônios
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article