Dag-1 carcinoma cell in studying the mechanisms of progression and therapeutic resistance in bladder cancer.
Eur Urol
; 39(3): 343-8, 2001 Mar.
Article
em En
| MEDLINE
| ID: mdl-11275731
ABSTRACT
OBJECTIVE:
We describe a new human bladder carcinoma cell line (DAG-1) established from a resected bladder cancer fragment and maintained in culture for more than 5 years and over 300 passages. METHODS ANDRESULTS:
Immunological, biochemical and molecular analysis showed that the DAG-1 cells (62 chromosomes) express the cytokeratines 8, 13, 18 and 20 that confirm their epithelial origin as well as numerous cytokine and cytokine receptor mRNAs. They secrete tissue-type plasminogen activator (t-PA), urokinase-type plasminogen activator (u-PA), plasminogen activator inhibitors (PAI-1 and PAI-2), and express u-PA receptors (u-PAR/CD87) at their surface. DAG-1 cells are resistant to TNFalpha- and IFNgamma-induced apoptosis, two cytokines secreted in the urine of Calmette-Guérin bacillus-treated patients and involved in the tumor regression.CONCLUSION:
The DAG-1 cell line is a useful tool, both in vitro and in vivo, to study the progression of bladder tumors and their mechanisms of resistance to immunotherapy in relation with PAI-2 and antioxidant enzymes.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Bexiga Urinária
/
Células Tumorais Cultivadas
/
Carcinoma de Células de Transição
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article