T cytotoxic 1 and T cytotoxic 2 CD8 T cells both inhibit IgE responses.
Int Arch Allergy Immunol
; 124(1-3): 187-9, 2001.
Article
em En
| MEDLINE
| ID: mdl-11306964
ABSTRACT
BACKGROUND:
It is well recognized that CD8 T cells inhibit IgE responses. In this study, we investigated the mechanism of CD8 T cell-mediated IgE suppression by comparing the capacity of T cytotoxic 1 (Tc1) and T cytotoxic 2 (Tc2) CD8 T cells to inhibit IgE responses to ovalbumin (OVA).METHODS:
Tc1 and Tc2 CD8 T cells were generated from OVA(257-264)-specific Vbeta5.2 T cell receptor (TcR) transgenic mice by stimulation with anti-CD3 and anti-CD28 under Tc1 and Tc2 polarizing conditions. Tc1 and Tc2 Vbeta5.2 TcR CD8 T cells (10(6)) were adoptively transferred to syngeneic mice, and following immunization with 100 micro of OVA/alum, serum IgE antibodies were measured by passive cutaneous anaphylaxis and expressed as the highest dilution that gave a detectable skin response.RESULTS:
Both Tc1 and Tc2 CD8 T cells from OT-I mice inhibited IgE.CONCLUSION:
Both Tc1 and Tc2 CD8 T cells promote Th1 immunity and inhibit IgE responses. This process appears to be independent of CD8 T cell-derived IFN-gamma, as both Tc2 (IFN-gamma-) and Tc1 (IFN-gamma+) CD8 T cells inhibited IgE.
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Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina E
/
Linfócitos T Citotóxicos
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article