Immunohistochemistry of bone sialoprotein and osteopontin during reparative dentinogenesis in vivo.
Chin J Dent Res
; 3(2): 38-43, 2000 Aug.
Article
em En
| MEDLINE
| ID: mdl-11314517
ABSTRACT
OBJECTIVE:
To investigate the stage-specific and tissue expression of bone sialoprotein (BSP) and osteopontin (OPN) during reparative dentinogenesis in vivo.METHODS:
Direct pulp-capping with Ca(OH)2 developed a model used for the investigation of reparative dentinogenesis in the exposed dental pulp. In this model, standardized class V cavities were prepared close to the gingival margin on the buccal surface of each tooth. Animals were sacrificed 3, 7, and 10 days post-operation. Immunohistochemical staining determined the tissue-specific expression of BSP and OPN during the process.RESULTS:
Odontoblast-like cells and reparative dentin reacted positively with BSP and OPN. The expression of BSP reached a peak at day 7, then gradually decreased, while the expression of OPN showed no significant difference between day 7 and day 10.CONCLUSION:
BSP and OPN may play different roles in reparative dentinogenesis. BSP may serve as a nucleator of hydroxyapatite crystal formation.
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Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Sialoglicoproteínas
/
Dentina Secundária
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article