Molecular cloning and analysis of the 5'-flanking region of the human MYPT1 gene.
Biochim Biophys Acta
; 1517(3): 424-9, 2001 Feb 16.
Article
em En
| MEDLINE
| ID: mdl-11342221
ABSTRACT
We have cloned and sequenced a 5 kb genomic fragment in the 5'-flanking region of the human myosin phosphatase target subunit 1. The transcription initiation site (+1) was 268 bp upstream from the translation start site. In this promoter there are no canonical TATA or CAAT box elements but there is a high GC-rich sequence. Basal promoter activity was due to the GC-rich region that contained one Sp1 transcription factor binding site, thus demonstrating that the MYPT1 gene is a housekeeping gene. Luciferase reporter assays showed the presence of two regions for positive elements and one for a negative element.
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Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas Fosfatases
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article