Binding of dimeric aminoglycosides to the HIV-1 rev responsive element (RRE) RNA construct.
Bioorg Med Chem Lett
; 11(9): 1127-31, 2001 May 07.
Article
em En
| MEDLINE
| ID: mdl-11354359
ABSTRACT
Through a series of elegant fluorescence measurements, particularly through stopped-flow kinetic measurements, it was recently demonstrated that aminoglycoside antibiotics are able to bind to the HIV-1 Rev responsive element (RRE) RNA construct in more than a 11 stoichiometry (Lacourciere, K. A.; Stivers, J. T.; Marino, J. P. Biocheminstry 2000, 39, 5630). Here, we present the binding study results of dimeric neomycin ligands through fluorescence anisotropy studies, to the HIV-1 RRE RNA construct. The dimeric neomycin molecules are observed to be able to bind the HIV-1 RRE RNA construct approximately 17-fold higher when compared to the monomeric neomycin, lending evidence that there are indeed two or more neomycin binding sites within the HIV-1 RRE construct.
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Base de dados:
MEDLINE
Assunto principal:
RNA Viral
/
HIV-1
/
Elementos de Resposta
/
Antibacterianos
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article