Bmx tyrosine kinase has a redundant function downstream of angiopoietin and vascular endothelial growth factor receptors in arterial endothelium.
Mol Cell Biol
; 21(14): 4647-55, 2001 Jul.
Article
em En
| MEDLINE
| ID: mdl-11416142
ABSTRACT
The Bmx gene, a member of the Tec tyrosine kinase gene family, is known to be expressed in subsets of hematopoietic and endothelial cells. In this study, mice were generated in which the first coding exon of the Bmx gene was replaced with the lacZ reporter gene by a knock-in strategy. The homozygous mice lacking Bmx activity were fertile and had a normal life span without an obvious phenotype. Staining of their tissues using beta-galactosidase substrate to assess the sites of Bmx expression revealed strong signals in the endothelial cells of large arteries and in the endocardium starting between days 10.5 and 12.5 of embryogenesis and continuing in adult mice, while the venular endothelium showed a weak signal only in the superior and inferior venae cavae. Of the five known endothelial receptor tyrosine kinases tested, activated Tie-2 induced tyrosyl phosphorylation of the Bmx protein and both Tie-2 and vascular endothelial growth factor receptor 1 (VEGFR-1) stimulated Bmx tyrosine kinase activity. Thus, the Bmx tyrosine kinase has a redundant role in arterial endothelial signal transduction downstream of the Tie-2 and VEGFR-1 growth factor receptors.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Tirosina Quinases
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Endotélio Vascular
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Glicoproteínas de Membrana
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Transdução de Sinais
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Proteínas Proto-Oncogênicas
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Receptores Proteína Tirosina Quinases
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Proteínas de Neoplasias
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article