An animal model to assess aversion to intra-oral capsaicin: increased threshold in mice lacking substance p.
Chem Senses
; 26(5): 491-7, 2001 Jun.
Article
em En
| MEDLINE
| ID: mdl-11418494
ABSTRACT
Despite the widespread consumption of products containing chemicals that irritate the oral mucosa, little is known about the underlying neural mechanisms nor is there a corresponding animal model of oral irritation. We have developed a rodent model to assess aversion to capsaicin in drinking water, using a paired preference paradigm. This method was used to test the hypothesis that the neuromodulator substance P (SP) plays a role in the detection of intra-oral capsaicin. 'Knockout' (KO) mice completely lacking SP and neurokinin A due to a disruption of the preprotachykinin A gene and a matched population of wild-type (WT) mice had free access to two drinking bottles, one containing water and the other capsaicin at various concentrations. Both KO and WT mice showed a concentration-dependent aversion to capsaicin. KO mice consumed significantly more capsaicin than WT at a single near threshold (1.65 microM) concentration, indicating that SP plays a limited role in the detection and rejection of oral irritants.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Limiar Gustativo
/
Capsaicina
/
Neurocinina A
/
Substância P
/
Camundongos Knockout
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article