Molecular cloning of silkworm paralytic peptide and its developmental regulation.
Biochem Biophys Res Commun
; 286(1): 67-73, 2001 Aug 10.
Article
em En
| MEDLINE
| ID: mdl-11485309
ABSTRACT
The silkworm paralytic peptide (PP) is a member of the ENF peptide family that exerts multiple biological activities involved in defense reaction and growth regulation. We isolated its cDNA and examined mRNA expression profiles. cDNA encoded 131 amino acids from which the 23-residue PP sequence was found at the C-terminal portion. Immunoblot analysis and paralytic activity assay indicated that inactive pro-protein in larval hemolymph was processed into active peptide immediately after bleeding. In the last larval instar, 0.6-kb PP mRNA was expressed in various tissues, of which the fat body was predominant. Its expression in the fat body decreased during the feeding period and then increased during metamorphic process. Juvenile hormone and 20-hydroxyecdysone upregulated its expression. At the embryonic stage, 1.5-kb mRNA, in addition to 0.6-kb mRNA, was expressed from 1 day after oviposition to hatching. PP was thus expressed stage-specifically under hormonal control.
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Base de dados:
MEDLINE
Assunto principal:
Neuropeptídeos
/
Regulação da Expressão Gênica no Desenvolvimento
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article