Human hepatoma cell telomerase activity inhibition and cell cycle modulation by its RNA component antisense oligodeoxyribonucleotides.
Acta Pharmacol Sin
; 21(8): 742-6, 2000 Aug.
Article
em En
| MEDLINE
| ID: mdl-11501185
ABSTRACT
AIM:
To investigate the effects of human telomerase RNA component antisense oligodeoxyribonucleotides on telomerase activity of human hepatoma cells and their effects on cell cycle distribution.METHODS:
Modified telomeric repeat amplification protocol was used to detect telomerase activity. Cell cycle was analyzed by flow cytometer.RESULTS:
Telomerase activity was detected in all of four human hepatoma cell lines but absent in normal liver cells. Antisense oligomers to human telomerase RNA component (hTR) inhibited telomerase activity of BEL-7404 human hepatoma cells markedly in vitro. After in vitro treatment with antisense oligomers for 96 h, cell cycle of BEL-7404 human hepatoma cells was mainly arrested at G2/M phase.CONCLUSION:
Antisense oligomers to hTR inhibited telomerase activity of BEL-7404 human hepatoma cells in vitro and resulted in cell cycle arrest at G2/M phase.
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Base de dados:
MEDLINE
Assunto principal:
Carcinoma Hepatocelular
/
Telomerase
/
Oligodesoxirribonucleotídeos Antissenso
/
Neoplasias Hepáticas
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article