Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution.
Curr Opin Biotechnol
; 12(4): 371-5, 2001 Aug.
Article
em En
| MEDLINE
| ID: mdl-11551465
ABSTRACT
With the advent of directed evolution techniques, protein engineering has received a fresh impetus. Engineering proteins for thermostability is a particularly exciting and challenging field, as it is crucial for broadening the industrial use of recombinant proteins. In addition to directed evolution, a variety of partially successful rational concepts for engineering thermostability have been developed in the past. Recent results suggest that amino acid sequence comparisons of mesophilic proteins alone can be used efficiently to engineer thermostable proteins. The potential benefits of the underlying, semirational 'consensus concept' are compared with those of rational design and directed evolution approaches.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Desenho de Fármacos
/
Alinhamento de Sequência
/
Evolução Molecular Direcionada
/
Estabilidade de Medicamentos
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article