Comparative studies on [Ca2+]i-level of fibroblasts from Alzheimer patients and control individuals.
Neurochem Res
; 26(7): 817-20, 2001 Jul.
Article
em En
| MEDLINE
| ID: mdl-11565613
ABSTRACT
The accumulation of the beta-amyloid peptide (betaAP) in the brain, produced from the ubiquitously expressed amyloid precursor protein (APP) is a defining feature of Alzheimer's disease (AD). Consistent with studies demonstrating the importance of skin biopsy in the diagnosis of neurodegenerative disorders, we investigated whether differences in intracellular free calcium levels ([Ca2+]i) of cultured cutaneous fibroblasts derived from sporadic AD patients and from age-matched control individuals might be present. [Ca2+]i was measured in Fura-2AM-loaded human fibroblasts by dual wavelength spectrofluorimetry. AD cells exhibited lower [Ca2+]i as compared to the control cultures. Exposure of fibroblasts to betaAP resulted in increased [Ca2+]i of the control cells, but not of AD fibroblasts. Our test could prove useful in supporting the diagnosis of (sporadic) AD in patients suspected of suffering from the disease.
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Base de dados:
MEDLINE
Assunto principal:
Cálcio
/
Fura-2
/
Doença de Alzheimer
/
Fibroblastos
/
Membranas Intracelulares
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article