Gene-trap mutagenesis: past, present and beyond.
Nat Rev Genet
; 2(10): 756-68, 2001 Oct.
Article
em En
| MEDLINE
| ID: mdl-11584292
ABSTRACT
Although at least 35,000 human genes have been sequenced and mapped, adequate expression or functional information is available for only approximately 15% of them. Gene-trap mutagenesis is a technique that randomly generates loss-of-function mutations and reports the expression of many mouse genes. At present, several large-scale, gene-trap screens are being carried out with various new vectors, which aim to generate a public resource of mutagenized embryonic stem (ES) cells. This resource now includes more than 8,000 mutagenized ES-cell lines, which are freely available, making it an appropriate time to evaluate the recent advances in this area of genomic technology and the technical hurdles it has yet to overcome.
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Base de dados:
MEDLINE
Assunto principal:
Mutagênese Insercional
/
Camundongos Mutantes
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article