Two-photon excitation fluorescence microscopy.
Annu Rev Biomed Eng
; 2: 399-429, 2000.
Article
em En
| MEDLINE
| ID: mdl-11701518
ABSTRACT
Two-photon fluorescence microscopy is one of the most important recent inventions in biological imaging. This technology enables noninvasive study of biological specimens in three dimensions with submicrometer resolution. Two-photon excitation of fluorophores results from the simultaneous absorption of two photons. This excitation process has a number of unique advantages, such as reduced specimen photodamage and enhanced penetration depth. It also produces higher-contrast images and is a novel method to trigger localized photochemical reactions. Two-photon microscopy continues to find an increasing number of applications in biology and medicine.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Microscopia de Fluorescência
Idioma:
En
Ano de publicação:
2000
Tipo de documento:
Article