Protein-DNA interaction and CpG methylation at rep*/vIL-10p of latent Epstein-Barr virus genomes in lymphoid cell lines.
Biol Chem
; 382(10): 1411-9, 2001 Oct.
Article
em En
| MEDLINE
| ID: mdl-11727824
ABSTRACT
The viral interleukin-10 promoter (vIL-10p), overlapping the rep* element in the Epstein-Barr virus (EBV) genome, is a promoter element active mostly in the late phase of the lytic cycle and immediately upon infection of B cells. rep* was, through transfection experiments with small plasmids, characterised as a cis element supporting oriP replicative function. In this study, in vivo protein binding and CpG methylation at rep*/vIL-10p were analysed in five cell lines that harbour strictly latent EBV genomes. Contrary to the invariably unmethylated dyad symmetry element (DS) of oriP, rep*/vIL-10p was highly methylated and showed only traces of protein binding in all examined cell lines. This result is in agreement with vIL-10p being an inactive promoter of EBV genomes, and makes it less likely that rep* functions as a replicative element of latent EBV genomes.
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Linfócitos
/
Proteínas
/
Interleucina-10
/
Herpesvirus Humano 4
/
Ilhas de CpG
/
Metilação de DNA
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article