A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival.
Immunity
; 15(5): 763-74, 2001 Nov.
Article
em En
| MEDLINE
| ID: mdl-11728338
ABSTRACT
The role of DNA methylation and of the maintenance DNA methyltransferase Dnmt1 in the epigenetic regulation of developmental stage- and cell lineage-specific gene expression in vivo is uncertain. This is addressed here through the generation of mice in which Dnmt1 was inactivated by Cre/loxP-mediated deletion at sequential stages of T cell development. Deletion of Dnmt1 in early double-negative thymocytes led to impaired survival of TCRalphabeta(+) cells and the generation of atypical CD8(+)TCRgammadelta(+) cells. Deletion of Dnmt1 in double-positive thymocytes impaired activation-induced proliferation but differentially enhanced cytokine mRNA expression by naive peripheral T cells. We conclude that Dnmt1 and DNA methylation are required for the proper expression of certain genes that define fate and determine function in T cells.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Metilação de DNA
/
DNA (Citosina-5-)-Metiltransferases
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article