A new analytical scale DNA affinity binding assay for analyses of specific protein-DNA interactions.
Anal Biochem
; 299(2): 203-10, 2001 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-11730344
ABSTRACT
We describe a rapid analytical assay for identification of proteins binding to specific DNA sequences. The DAPSTER assay (DNA affinity preincubation specificity test of recognition assay) is a DNA affinity chromatography-based microassay that can discriminate between specific and nonspecific protein-DNA interactions. The assay is sensitive and can detect protein-DNA interactions and larger multicomponent complexes that can be missed by other analytical methods. Here we describe in detail the optimization and utilization of the DAPSTER assay to isolate AP-1 complexes and associated proteins in multimeric complexes bound to the AP-1 DNA element.
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Cromatografia de Afinidade
/
Receptores de Superfície Celular
/
Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article