Interaction of lidocaine with reactive oxygen and nitrogen species.
Eur J Anaesthesiol
; 18(12): 816-22, 2001 Dec.
Article
em En
| MEDLINE
| ID: mdl-11737181
ABSTRACT
BACKGROUND AND OBJECTIVE:
To investigate the ability of lidocaine to inhibit reactive oxygen and/or nitrogen species generation by either human leukocytes or cell-free systems via luminol- and lucigenin-enhanced chemiluminescence.METHODS:
Venous blood was obtained from healthy volunteers and leukocytes were isolated, from which chemiluminescence was generated. Also, chemiluminescence, induced by H(2)O(2), HOCl, peroxynitrite or ferrous iron, was generated in cell-free systems.RESULTS:
Lidocaine produced a concentration-dependent inhibition in chemiluminescence generated by leukocytes (92 +/- 1%, 1 mM). In cell-free experiments, lidocaine (1 mM) markedly inhibited chemiluminescence of xanthine-xanthine oxidase (24 +/- 3%), while it slightly suppressed hypochlorous acid-induced chemiluminescence (9 +/- 2%). Peroxynitrite-induced luminol- and lucigenin-enhanced chemiluminescence were also inhibited by lidocaine at 1 mM (19 +/- 3% and 48 +/- 8%, respectively). Lidocaine did not affect chemiluminescence generated by FeSO(4). However, lidocaine produced a biphasic effect on H(2)O(2)-induced chemiluminescence (37 +/- 5% inhibition at 0.01 mM and 61 +/- 17% activation at 1 mM).CONCLUSIONS:
Lidocaine can elicit direct scavenging activity at high concentrations that might be important at or near the site of injection in local anaesthetic use.
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Base de dados:
MEDLINE
Assunto principal:
Espécies Reativas de Oxigênio
/
Anestésicos Locais
/
Leucócitos
/
Lidocaína
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article