Heteromeric AMPA receptors assemble with a preferred subunit stoichiometry and spatial arrangement.
Neuron
; 32(5): 841-53, 2001 Dec 06.
Article
em En
| MEDLINE
| ID: mdl-11738030
ABSTRACT
AMPA receptors are thought to be a tetrameric assembly of the subunits GluR1-4. We have examined whether two coexpressed subunits (GluR1/2) combine at random to form channels, or preferentially assemble with a specific stoichiometry and spatial configuration. The subunits carried markers controlling ion permeation and desensitization, and these properties were monitored as a function of relative expression level and subunit composition. Homomeric receptors assembled stochastically while heteromeric receptors preferentially formed with a stoichiometry of two GluR1 and two GluR2 subunits, and with identical subunits positioned on opposite sides of the channel pore. This structure will predominate if GluR1 binds to GluR2 more rapidly during receptor assembly than other subunit combinations. The practical outcome of selective heteromeric assembly is a more homogenous receptor population in vivo.
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Base de dados:
MEDLINE
Assunto principal:
Receptores de AMPA
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article