The effects of platelet apheresis in total hip replacement surgery on platelet activation.
Acta Anaesthesiol Scand
; 46(1): 68-73, 2002 Jan.
Article
em En
| MEDLINE
| ID: mdl-11903075
ABSTRACT
BACKGROUND:
Autologous platelet rich plasma (PRP) harvest with autotransfusion devices has been used for 10 years in cardiac surgery and recently in orthopedics as a blood saving method. The quality of the harvested platelets has not been adequately examined, in part because of methodological difficulties in studying platelet function during surgery.METHODS:
Twenty patients undergoing primary total hip replacement (THR) were studied. Ten patients underwent an immediate preoperative platelet apheresis to obtain concentrated platelet rich plasma (c-PRP). The other 10 patients not undergoing apheresis were allocated to a control group. Platelet activation was evaluated as the population expressing P-selectin on the surface of platelets in the c-PRP and in blood samples collected pre-, per- and postoperatively. The method used was flow cytometry. RESULTS ANDCONCLUSIONS:
A minor population of activated platelets was found to be circulating in the patients' blood, with a highly significant difference between patients (P = 0.005), and with a range of 1-23% in peroperative activation. PRP harvest did not significantly alter platelet activity. The platelet apheresis procedure did not inhibit platelet function in the c-PRP, as judged by a high proportion of platelets that could be activated in ADP stimulation experiments (mean value +/- SD 86% +/- 7.5%).
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Base de dados:
MEDLINE
Assunto principal:
Ativação Plaquetária
/
Plaquetoferese
/
Artroplastia de Quadril
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article