Noninvasive genotyping of dopamine receptor D4 (DRD4) using nanograms of DNA from substance-dependent patients.
Curr Med Chem
; 9(8): 793-7, 2002 Apr.
Article
em En
| MEDLINE
| ID: mdl-11966444
A noninvasive DNA sampling method has been implemented collecting buccal mucosa cells by cotton wool swabs. An amount of 0.2 2 microg DNA per patient was obtained after the phenol-extraction procedure and 0.2 2 ng DNA template was sufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopamine receptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separation by laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drug-dependent subjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variants beside the common 4-repeat DRD4 allele.
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Base de dados:
MEDLINE
Assunto principal:
Receptores de Dopamina D2
/
Transtornos Relacionados ao Uso de Substâncias
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article