Superimposition and Analysis of ESI Tandem Mass Spectra.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
; 33(6): 665-670, 2001.
Article
em En
| MEDLINE
| ID: mdl-12035059
ABSTRACT
Methionine enkephalin and four synthetic peptides (a heptapeptide and its modified form, a decapeptide and an icosapeptide) were fragmented using ESI tandem mass spectrometry with certain varying instrumental parameters, such as amplitude of excitation, to produce a series of MS/MS spectra. Using the Bruker DataAnalysis software, some complementary MS/MS spectra were chosen to form a superimposed MS/MS spectrum with consecutive triplets (as well as a few doublets) consisting of product ions AnBnCn or X n Y n Z n. The amino acid sequences of the peptides were easily and accurately determined from the superimposed MS/MS spectrum, suggesting that the method is easy and suitable for peptide sequencing by tandem mass spectrometry.
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Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article