Phenylethylamine induces an increase in cytosolic Ca2+ in yeast.
Biosci Biotechnol Biochem
; 66(5): 1069-74, 2002 May.
Article
em En
| MEDLINE
| ID: mdl-12092817
ABSTRACT
Beta-phenylethylamine (PEA) induced an increase in cytosolic free calcium ion concentration ([Ca2+]c) in Saccharomyces cerevisiae cells monitored with transgenic aequorin, a Ca2+-dependent photoprotein. The PEA-induced [Ca2+]c increase was dependent on the concentrations of PEA applied, and the Ca2+ mostly originated from an extracellular source. Preceding the Ca2+ influx, H2O2 was generated in the cells by the addition of PEA. Externally added H2O2 also induced a [Ca2+]c increase. These results suggest that PEA induces the [Ca2+]c increase via H2O2 generation. The PEA-induced [Ca2+]c increase occurred in the mid1 mutant with a slightly smaller peak than in the wild-type strain, indicating that Mid1, a stretch-activated nonselective cation channel, may not be mainly involved in the PEA-induced Ca2+ influx. When PEA was applied, the MATa mid1 mutant was rescued from alpha-factor-induced death in a Ca2+-limited medium, suggesting that the PEA-induced [Ca2+]c increase can reinforce calcium signaling in the mating pheromone response pathway.
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Base de dados:
MEDLINE
Assunto principal:
Fenetilaminas
/
Saccharomyces cerevisiae
/
Cálcio
/
Citosol
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article