Multiplex RT-PCR assay for the detection of major fusion transcripts in Taiwanese children with B-lineage acute lymphoblastic leukemia.
Med Pediatr Oncol
; 39(1): 12-7, 2002 Jul.
Article
em En
| MEDLINE
| ID: mdl-12116073
ABSTRACT
BACKGROUND:
The classification of B-lineage acute lymphoblastic leukemia (ALL) by specific chromosomal translocations may have prognostic implications. Reverse transcriptase-polymerase chain reaction (RT-PCR) assay is a useful tool for the detection of fusion transcript resulting from specific chromosomal translocation of the leukemic cells. In general, fusion transcripts are determined individually, a process which is labor intensive in order to detect all major fusion transcripts. PROCEDURE We use a multiplex RT-PCR assay to detect both the CML- and ALL-type BCR-ABL transcripts of the t(9;22), all described variants of the E2A-PBX1 transcripts of t(1;19), the MLL-AF4 transcripts of t(4;11), and all described variants of TEL-AML1 (also termed ETV6-CBFA2) of the cryptic t(12;21) in 165 leukemic samples at diagnosis.RESULTS:
The study yielded a completely concordant result with those obtained by the individual RT-PCR assay. In this cohort of Taiwan children, the relative frequencies of the four translocations of B-lineage ALL were as following 6% with ALL-type t(9;22)/BCR-ABL, 7% t(1;19)/E2A-PBX1, 3% t(4;11)/MLL-AF4, and 18% t(12;21)/TEL-AML1, comparable to those in the Western countries.CONCLUSION:
Multiplex RT-PCR assay is an efficient, sensitive, accurate, and cost-effective diagnostic tool, which will likely improve our ability in accurately and rapidly risk-stratifying children with ALL.
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Base de dados:
MEDLINE
Assunto principal:
Biomarcadores Tumorais
/
Proteínas de Fusão Oncogênica
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Proteínas de Fusão bcr-abl
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Linfoma de Burkitt
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Proteínas de Homeodomínio
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Leucemia-Linfoma Linfoblástico de Células Precursoras
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Proteínas de Neoplasias
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article